Safranin O

This is used for the detection of cartilage, mucin, and mast cell granules on formalin-fixed, paraffin-embedded tissue sections, and may be used for frozen sections as well. The cartilage and mucin stain orange to red, and the nuclei stain black. The background is stained green.

Tissue: Formalin fixed, paraffin embedded sections.

It is also used in the staining protocol of Prussian Blue.